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SHIBIN SHAFI

Biotechnology graduate · QA/QC and R&D aspiring researcher

Palakkad, Kerala, India

@shibin_shafi

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B.Tech, Biotechnology and Biochemical Engineering at JCT College of Engineering and Technology · Graduating 2026

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About

Biotechnology and Biochemical Engineering graduate with hands-on research experience in microbiology, molecular biology, and mammalian cell culture. Seeking entry-level opportunities in QA, QC, R&D, and laboratory research within biotechnology and pharmaceutical industries.

Experience

  • Research Intern - Immunology & Molecular Biology
    National Institute of Technology Calicut (NITC) · Kozhikode
    Jan 2026 – 2026

    Maintained THP-1 human monocytic cells and performed PMA-induced differentiation into macrophage-like cells. Conducted host-pathogen infection assays involving Klebsiella pneumoniae and performed CFU-based bacterial load estimation. Performed bacterial culture and growth-curve analysis using microbiological laboratory techniques. Applied molecular biology techniques, including RNA isolation, cDNA preparation, PCR/qPCR optimisation, and agarose gel electrophoresis. Gained practical experience in laboratory procedures, experimental workflows, and scientific data handling.

  • Intern — Molecular Biology & Microbial Diagnostics
    Veta Genomics · Thrissur
    Jul 2025 – 2025

    Performed bacterial DNA extraction and DNA quantification following laboratory procedures and GLP principles. Worked on PCR primer design and microbial DNA analysis for diagnostic applications. Gained practical exposure to microbial sample handling, aseptic techniques, and laboratory documentation.

Education

Skills

1 to 2 years 1 to 2 years A year or two of steady use.
PCR qPCR Cell Culture Microbiology RNA isolation Molecular Biology Aseptic techniques DNA quantification
6 to 12 months 6 to 12 months Most of a year behind them.
Primer Design DNA Extraction
<3 months <3 months Just getting started - under three months. This is also what shows when a level has not been set.
GMP GLP Python Data Analysis Record Keeping Laboratory safety Media Preparation Laboratory Techniques Laboratory Documentation Agarose Gel Electrophoresis Standard Operating Procedures

Tools / apps / platforms

<3 months <3 months Just getting started - under three months. This is also what shows when a level has not been set.
Microsoft Excel Microsoft Office

Languages

Not stated Not stated No level given for this one.
Tamil Speak Hindi Speak English Speak Malayalam Speak

Projects

  • Molecular Biology Techniques (Workshop)
    DNA Isolation, PCR

    Practical training in DNA isolation and PCR, covering sample handling, amplification, and result verification.

  • Gene Regulation Study in THP-1-Derived Human Macrophages upon Klebsiella pneumoniae Infection
    Cell Culture, Molecular Biology

    Investigated host immune gene regulation and TLR-associated gene expression in THP-1-derived macrophages during Klebsiella pneumoniae infection, gaining hands-on experience in mammalian cell culture, infection assays, molecular biology techniques, and host-pathogen interactions.

Courses & certifications

  • Honors degree in Agrochemicals, Medical Waste Treatment, Drug Design and Discovery, Plant Tissue Culture, Pathology and Microbiology, Industrial Effluent Treatment
  • Research Internship Certificate · National Institute of Technology Calicut
  • Cell Biology: Cellular Organization, Division and Processes · NPTEL / SWAYAM

🏆 Achievements & awards

  • Poster Presentation — Second Runner-Up · 2025

    International Conference on Bionexus Towards Global Stability (Evogen'25), Karunya Institute of Technology and Science, Coimbatore.

🎯 Hobbies & interests

  • Cancer Immunology
  • Applied Immunology

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