Madhumitha R

About

Stem Cell Technology and Regenerative Medicine researcher with hands-on laboratory experience in hESC/iPSC culture, stem cell differentiation, and molecular and cellular assay development. Trained in RNA/DNA isolation, cDNA synthesis, PCR/qPCR, flow cytometry, immunofluorescence, and confocal microscopy, with a focus on quality control and reproducibility.

Experience

• Observership Intern
  Bioplus Lifesciences Pvt. Ltd. · Bengaluru, India
  Jul 2023 – Oct 2023

  Structured observership across multiple instrument stations and departments at a pharmaceutical company; documented GLP, biosafety protocols, and laboratory quality control standards; shadowed pharma R&D and production departments.

Education

• M.Sc.
  Manipal Academy of Higher Education
  Stem Cell Technology and Regenerative Medicine · Aug 2024 – Jun 2026
• B.Sc.
  Garden City University
  Biotechnology, Biochemistry, Genetics · Nov 2021 – May 2024

Skills

• Data Analysis 6 to 12 months
• Good Laboratory Practices 6 to 12 months
• DNA Extraction 6 to 12 months
• PCR 6 to 12 months
• Laboratory Quality Control 6 to 12 months
• Research Methodology 6 to 12 months
• qPCR 6 to 12 months
• Scientific Documentation 6 to 12 months
• Aseptic techniques 6 to 12 months
• Immunofluorescence 6 to 12 months
• RNA isolation 6 to 12 months
• Biosafety 6 to 12 months
• Cell culture techniques 6 to 12 months

Tools / apps / platforms

• ImageJ 6 to 12 months
• Microsoft Excel 6 to 12 months
• Microsoft PowerPoint 6 to 12 months
• Microsoft Word 6 to 12 months

Languages

• English Fluent Speak · Read · Write
• Hindi Conversational Speak · Read · Write
• Kannada Conversational Speak
• Tamil Native / Bilingual Speak · Read · Write
• Telugu Conversational Speak

Projects

• Major Project — Spindle Assembly Checkpoint (SAC) Pathway Analysis
  hESC, iPSC, HUES, RNA isolation, cDNA synthesis, qPCR, flow cytometry, immunofluorescence

  Designed and executed an experimental study of the spindle assembly checkpoint pathway using in vitro cell models; cultured and passaged hESC, iPSC, and HUES lines; performed RNA isolation, cDNA synthesis, qPCR, flow cytometry analysis, and immunofluorescence.

• Minor Project — Induction of Naïve Pluripotency Using Induction Media
  iPSC, immunofluorescence, PCR, cell viability assays

  Induced naïve pluripotency in an iPSC line using optimized induction media; monitored morphology, characterized cell state, evaluated pluripotency markers, and optimized protocols through troubleshooting.

Courses & certifications

• Biorujivith International Conference · Garden City University · 2024

🤝 Volunteering

• Biorujivith International Conference · Volunteer · 2022