Dipshikha Yadav

About

Biotechnology graduate with hands-on industry and research experience in biopharmaceutical R&D, including antibody phage display, binder screening, protein expression, and molecular biology techniques. Seeking to transition into bioinformatics and computational biology through structured learning in NGS analysis, sequence analysis, and Python/R-based workflows.

Experience

• Role
  Yashraj Biotechology LTD
  Jun 2025 – Sep 2026

  Executed antibody phage display workflows for target-specific binder identification, including library amplification, iterative biopanning, clone screening, and molecular validation. Performed three rounds of biopanning against multiple targets, including Panacea, vancomycin, cardiolipin, Tg, and TSHR, screening >200 clones per target using phage ELISA. Investigated repeated false-positive binders for Tg and identified biotinylation as a key factor in improving specificity; identified 143 potential positive binders for sequencing. Performed plasmid extraction and prepared >500 positive/enriched clones for sequencing-based validation. Performed Western blot analysis and handled M13KO7 helper phage and SS320 E. coli under aseptic conditions. Maintained GLP/GDP-compliant documentation, inventory management, and resource coordination.

• Internship
  Institute of Chemical Technology · Mumbai
  Oct 2024 – Jan 2025

  Conducted HPLC, FTIR, and Kjeldahl analysis for qualitative characterization and formulation testing of cosmetic products, including shampoo and conditioner. Implemented enzyme immobilization techniques to produce partially hydrolyzed guar gum for industrial and cosmetic applications.

• Role
  Advanced Centre for Treatment Research & Education in Cancer (ACTREC) · Kharghar
  Jan 2024 – Sep 2024

  Investigated radiation-induced effects on HFSCs using C57BL/6 mice, including animal handling and radiation equipment operation. Analyzed stem cell proliferation and marker expression using RT-qPCR and interpreted gene expression results. Performed FACS isolation of stem cell populations and conducted immunofluorescence assays and H&E histological analysis.

• Internship
  Bioriidl
  Jul 2023 – Aug 2023

  Conducted a comparative study of the antibacterial and antioxidant activity of different honey samples. Utilized UV-Vis spectrophotometry and colorimetry to measure optical density and evaluate antioxidant activity. Cultured and maintained bacterial strains under sterile conditions, including media preparation.

• Internship
  MGM Medical College · Kamothe, Navi Mumbai
  Jul 2022 – Aug 2022

  Gained hands-on experience in RT-PCR, GeneXpert assay, and DNA/RNA extraction, along with foundational training in flow cytometry. Acquired practical knowledge of zebrafish breeding and maintenance, and performed bone and cartilage staining in zebrafish larvae.

Education

• 2024 AU
  BTech in Biotechnology

  Amity University Mumbai

  Biotechnology · Dec 2020 – Jun 2024

  Biotechnology · Dec 2020 – Jun 2024
• 2020 NH
  Higher Secondary School (Class XII)

  New Horizon Public School

  Jun 2018 – Jun 2020

  Jun 2018 – Jun 2020
• 2018 MI
  Senior Secondary School (Class X)

  Mahatma International School

  May 2018 – 2018

  May 2018 – 2018

Skills

Tools / apps / platforms

Languages

Courses & certifications

• Python for Genomic Data Science · John Hopkins / Coursera · 2026
• Introduction to Genomic Technologies · John Hopkins / Coursera · 2026
• Researcher’s Guide to Omics Data · Fred Hutch Cancer Center / Coursera · 2026
• Cell Culture and Translational Application online Workshop · Vidyothini Edutech Pvt. Ltd. · 2025
• Molecular Docking , Biological Software & Cell Culture Techniques Workshop · Satyabhama Institute of Science & Technology · 2025
• 10 Day Technical Hands-On Workshop in MD Simulations · BDG Lifesciences · 2025
• Bioinformatics Internship · Biotecnika · 2025
• AI and Machine Learning in Biology · Biotecnika · 2025
• Flow Cytometry Workshop · Bioriddl · 2023

🏆 Achievements & awards

• GATE Biotechnology Qualified · 2025
• NEET (UG) Qualified · 2020

⚽ Extracurricular activities

• Active Member, AmiSustain Club

  Contributed to environmental initiatives and positive impact projects; served on the Research and Content Team and Event Management Team.

• Volunteer, AmiBiome 2023

  Managed and planned the Hawkeye event.

• Participant, AmiVigyaan 2023

  Participated in multiple event activities.

• Millennium Fellowship Class of 2023

  Worked on the project 'Little Moments of Joy' focused on community engagement and improving well-being.